GSI RESEARCH PROTOCOLS

The GSI uses standard protocols and operating procedures relative to biological and operational/technical evaluations of treatment technologies to the greatest extent possible at each test scale (bench, land-based and shipboard). This consistency will maximize comparability and regulatory relevancy of the experimental results.

The standard operating procedures (SOPs), detailed below, will be subject to additional revision as needed over time to assure they maintain maximum effectiveness and consistency with any standard methods that may be generated by official sources.

GSI BENCH-SCALE SOPs
Sample Custody	Procedure for Labelling Bench-Scale Samples GSI/SOP/G/SCU/2
Culturing	Procedure for Culturing the Freshwater Rotifer Branchionus calyciflorus GSI/SOP/BS/C/1
	Procedure for Culturing the Freshwater Amphipod Hyalella azteca GSI/SOP/BS/C/2
	Procedure for Culturing the Cladocerans Daphnia magna and Ceriodaphnia dubia GSI/SOP/BS/C/3
Chemical Degradation	Procedure for Determining Chemical Degradation of a Primary Treatment or Its By-Products GSI/SOP/BS/CD/1
Chemistry	Procedure for Analyzing the Concentration of Ozone in Water GSI/SOP/BS/RA/C/1
	Procedure for Determining Total Residual Oxidants (TRO) in Water GSI/SOP/BS/RA/C/2
	Procedures for Measuring Organic Carbon in Aqueous Samples GSI/SOP/BS/RA/C/3
	Procedure for Determining Percent Transmittance (%T) in Water at 254 nm GSI/SOP/BS/RA/C/4
	Procedure for Measuring Organic Compounds using High Performance Liquid Chromatography (HPLC) GSI/SOP/BS/RA/C/5
	Procedure for Analyzing Total Residual Chlorine Concentrations in Water GSI/SOP/BS/RA/C/6
	Procedure for Analyzing Hydrogen Peroxide Concentrations in Water GSI/SOP/BS/RA/C/7
	Procedure for Analyzing Total Suspended Solids (TSS) GSI/SOP/BS/RA/C/8
	Procedure for pH Meter Calibration and pH Measurement for Ballast Treatment Systems using Basic pH as the Active Substance GSI/SOP/BS/RA/C/9
Dose Effectiveness	Procedure for Assessing Dose-Effectiveness of a Ballast Treatment System Using a Copepod GSI/SOP/BS/RA/DE/1
	Procedure for Assessing Dose-Effectiveness of a Ballast Treatment System Using the Daphnid Daphia magna GSI/SOP/BS/RA/DE/2
	Procedure for Assessing Dose-Effectiveness of a Ballast Treatment System Using the Freshwater Rotifer Branchionus calyciflorus GSI/SOP/BS/RA/DE/3
	Procedure for Assessing Dose Effectiveness of a Ballast Treatment System Using Cysts of the Freshwater Rotifer Branchionus calyciflorus GSI/SOP/BS/RA/DE/4
	Procedure for Assessing Dose Effectiveness of a Ballast Treatment System Using Selenastrum capricornutum GSI/SOP/BS/RA/DE/5
	Procedure for Exposing Test Organisms to a Ballast Treatment System Using Large Volume Influent and Effluent Tanks GSI/SOP/BS/RA/DE/6
Microbial Analysis	Procedure For Quantifying Heterotrophic Plate Counts (HPCs) Using IDEXX's SimPlate® for HPC Method GSI/SOP/BS/RA/MA/1
	Procedure for the Detection and Enumeration of Enterococcus using Enterolert™ GSI/SOP/BS/RA/MA/3
	Procedure for the Detection and Enumeration of Total Coliforms and E. coli Using IDEXX's Colilert® GSI/SOP/BS/RA/MA/4
Residual Toxicity	Procedure for Measuring Residual Toxicity Using Ceriodaphnia dubia GSI/SOP/BS/RA/RT/1
	Procedure for Measuring Residual Toxicity Using Fathead Minnows (Pimephales promelas) GSI/SOP/BS/RA/RT/2
	Procedure for Measuring Residual Toxicity Using the Alga Selenastrum capricornutum GSI/SOP/BS/RA/RT/3
	Procedure for Measuring Residual Toxicity Using the Amphipod Hyalella azteca GSI/SOP/BS/RA/RT/4
	Procedure for Measuring Residual Toxicity Using the Freshwater Rotifer Branchionus calyciflorus GSI/SOP/BS/RA/RT/5
	Procedure for Assessing Chronic Residual Toxicity of a Ballast Treatment System to Ceriodaphia dubia GSI/SOP/BS/RA/RT/6
	Procedure for Assessing Chronic Residual Toxicity of a Ballast Treatment System to the Fathead Minnow (Pimephales promelas) GSI/SOP/BS/RA/RT/7
	Procedure for Assessing Chronic Residual Toxicity of a Ballast Treatment System Using the Alga Selenastrum capricornutum GSI/SOP/BS/RA/RT/8
GSI LAND-BASED SOPs
Sample Custody	Procedure for Labeling Samples Collected at the GSI Land-Based RDTE Facility GSI/SOP/G/RA/SC/3
General	Procedure for Operating the GSI Land-Based RDTE Facility GSI/SOP/LB/G/O/1
	Procedure for Cleaning the Retention Tanks and Other Equipment at the GSI Land-Based RDTE Facility GSI/SOP/LB/G/O/3
	Procedure for Injecting Organisms and Solids into the GSI Land-Based RDTE Facility GSI/SOP/LB/G/O/5
	Procedure for Ensuring Worker Health and Safety at the GSI Land-Based RDTE Facility GSI/SOP/LB/G/S/1
Sample Collection	Procedure for Collecting Biological Sample Water Via In-Line Sample Ports GSI/SOP/LB/RA/SC/1
	Procedure for Algae/Small Protozoa Sample Collection GSI/SOP/LB/RA/SC/3
	Procedure for Microbial Sample Collection GSI/SOP/LB/RA/SC/4
	Procedure for Zooplankton Sample Collection GSI/SOP/LB/RA/SC/6
Sample Analysis	Procedure for Algae/Small Protozoan Sample Analysis GSI/SOP/LB/RA/SA/1
	Procedure for Zooplankton Sample Analysis GSI/SOP/LB/RA/SA/2

 

© Great Ships Initiative 2010.